LymphedemaV1, Main, Exploration, bibRecord, 006288

FGF-regulated BMP signaling is required for eyelid closure and to specify conjunctival epithelial cell fate

Identifieur interne : 006288 ( Main/Exploration ); précédent : 006287; suivant : 006289

FGF-regulated BMP signaling is required for eyelid closure and to specify conjunctival epithelial cell fate

Auteurs : Jie Huang [États-Unis] ; Lisa K. Dattilo [États-Unis] ; Ramya Rajagopal [États-Unis] ; Ying Liu [États-Unis] ; Vesa Kaartinen [États-Unis] ; Yuji Mishina [États-Unis] ; Chu-Xia Deng [États-Unis] ; Lieve Umans [Belgique] ; An Zwijsen [Belgique] ; Anita B. Roberts [États-Unis] ; David C. Beebe [États-Unis]

Source :

Development (Cambridge, England) [ 0950-1991 ] ; 2009.

RBID : PMC:2673764

Descripteurs français

KwdFr :
- Animaux, Animaux nouveau-nés, Cellules épithéliales (cytologie), Cellules épithéliales (métabolisme), Conjonctive (croissance et développement), Conjonctive (cytologie), Conjonctive (embryologie), Conjonctive (malformations), Différenciation cellulaire (physiologie), Facteurs de croissance fibroblastique (physiologie), Facteurs de transcription Forkhead (métabolisme), Follicule pileux (croissance et développement), Follicule pileux (embryologie), JNK Mitogen-Activated Protein Kinases (métabolisme), Paupières (croissance et développement), Paupières (embryologie), Paupières (malformations), Protéine Smad-4 (génétique), Protéine Smad-4 (métabolisme), Protéine morphogénétique osseuse de type 4 (biosynthèse), Protéines Hedgehog (métabolisme), Protéines Smad régulées par les récepteurs (génétique), Protéines morphogénétiques osseuses (physiologie), Récepteur FGFR2 (génétique), Récepteur FGFR2 (métabolisme), Récepteurs de la protéine morphogénique osseuse de type I (génétique), Souris, Souris knockout, Transduction du signal.
MESH :
- biosynthèse : Protéine morphogénétique osseuse de type 4.
- croissance et développement : Conjonctive, Follicule pileux, Paupières.
- cytologie : Cellules épithéliales, Conjonctive.
- embryologie : Conjonctive, Follicule pileux, Paupières.
- génétique : Protéine Smad-4, Protéines Smad régulées par les récepteurs, Récepteur FGFR2, Récepteurs de la protéine morphogénique osseuse de type I.
- malformations : Conjonctive, Paupières.
- métabolisme : Cellules épithéliales, Facteurs de transcription Forkhead, JNK Mitogen-Activated Protein Kinases, Protéine Smad-4, Protéines Hedgehog, Récepteur FGFR2.
- physiologie : Différenciation cellulaire, Facteurs de croissance fibroblastique, Protéines morphogénétiques osseuses.
- Animaux, Animaux nouveau-nés, Souris, Souris knockout, Transduction du signal.

English descriptors

KwdEn :
- Animals, Animals, Newborn, Bone Morphogenetic Protein 4 (biosynthesis), Bone Morphogenetic Protein Receptors, Type I (genetics), Bone Morphogenetic Proteins (physiology), Cell Differentiation (physiology), Conjunctiva (abnormalities), Conjunctiva (cytology), Conjunctiva (embryology), Conjunctiva (growth & development), Epithelial Cells (cytology), Epithelial Cells (metabolism), Eyelids (abnormalities), Eyelids (embryology), Eyelids (growth & development), Fibroblast Growth Factors (physiology), Forkhead Transcription Factors (metabolism), Hair Follicle (embryology), Hair Follicle (growth & development), Hedgehog Proteins (metabolism), JNK Mitogen-Activated Protein Kinases (metabolism), Mice, Mice, Knockout, Receptor, Fibroblast Growth Factor, Type 2 (genetics), Receptor, Fibroblast Growth Factor, Type 2 (metabolism), Signal Transduction, Smad Proteins, Receptor-Regulated (genetics), Smad4 Protein (genetics), Smad4 Protein (metabolism).
MESH :
- chemical , biosynthesis : Bone Morphogenetic Protein 4.
- chemical , genetics : Bone Morphogenetic Protein Receptors, Type I, Receptor, Fibroblast Growth Factor, Type 2, Smad Proteins, Receptor-Regulated, Smad4 Protein.
- chemical , metabolism : Forkhead Transcription Factors, Hedgehog Proteins, JNK Mitogen-Activated Protein Kinases, Receptor, Fibroblast Growth Factor, Type 2, Smad4 Protein.
- chemical , physiology : Bone Morphogenetic Proteins, Fibroblast Growth Factors.
- abnormalities : Conjunctiva, Eyelids.
- cytology : Conjunctiva, Epithelial Cells.
- embryology : Conjunctiva, Eyelids, Hair Follicle.
- growth & development : Conjunctiva, Eyelids, Hair Follicle.
- metabolism : Epithelial Cells.
- physiology : Cell Differentiation.
- Animals, Animals, Newborn, Mice, Mice, Knockout, Signal Transduction.

Abstract

Summary

There are conflicting reports about whether BMP signaling is required for eyelid closure during fetal development. This question was addressed using mice deficient in BMP or TGFβ signaling in prospective eyelid and conjunctival epithelial cells. Genes encoding two type I BMP receptors, the type II TGFβ receptor, two BMP- or two TGFβ-activated R-Smads or the co-Smad Smad4 were deleted from the ocular surface ectoderm using Cre recombinase. Only mice with deletion of components of the BMP pathway had an `eyelid open at birth' phenotype. Mice lacking Fgf10 or Fgfr2 also have open eyelids at birth. To better understand the pathways that regulate BMP expression and function during eyelid development, we localized BMPs and BMP signaling intermediates in Fgfr2 and Smad4 conditional knockout (CKO) mice. We found that Fgfr2 was required for the expression of Bmp4, the normal distribution of Shh signaling and for preserving the differentiation of the conjunctival epithelium. FGF signaling also promoted the expression of the Wnt antagonist Sfrp1 and suppressed Wnt signaling in the prospective eyelid epithelial cells, independently of BMP function. Transcripts encoding Foxc1 and Foxc2, which were previously shown to be necessary for eyelid closure, were not detectable in Smad4^CKO animals. c-Jun, another key regulator of eyelid closure, was present and phosphorylated in eyelid periderm cells at the time of fusion, but failed to translocate to the nucleus in the absence of BMP function. Smad4^CKO mice also showed premature differentiation of the conjunctival epithelium, conjunctival hyperplasia and the acquisition of epidermal characteristics, including formation of an ectopic row of hair follicles in place of the Meibomian glands. A second row of eyelashes is a feature of human lymphedema-distichiasis syndrome, which is associated with mutations in FOXC2.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2673764

DOI: 10.1242/dev.034082
PubMed: 19369394
PubMed Central: 2673764

Affiliations:

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">FGF-regulated BMP signaling is required for eyelid closure and to specify
 conjunctival epithelial cell fate</title>
<author><name sortKey="Huang, Jie" sort="Huang, Jie" uniqKey="Huang J" first="Jie" last="Huang">Jie Huang</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"> Department of Ophthalmology and Visual Sciences, Washington University, St Louis, MO 63130, USA.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">États-Unis</country>
<wicri:regionArea> Department of Ophthalmology and Visual Sciences, Washington University, St Louis, MO 63130</wicri:regionArea>
<wicri:noRegion>MO 63130</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Dattilo, Lisa K" sort="Dattilo, Lisa K" uniqKey="Dattilo L" first="Lisa K." last="Dattilo">Lisa K. Dattilo</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"> Department of Ophthalmology and Visual Sciences, Washington University, St Louis, MO 63130, USA.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">États-Unis</country>
<wicri:regionArea> Department of Ophthalmology and Visual Sciences, Washington University, St Louis, MO 63130</wicri:regionArea>
<wicri:noRegion>MO 63130</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Rajagopal, Ramya" sort="Rajagopal, Ramya" uniqKey="Rajagopal R" first="Ramya" last="Rajagopal">Ramya Rajagopal</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"> Department of Ophthalmology and Visual Sciences, Washington University, St Louis, MO 63130, USA.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">États-Unis</country>
<wicri:regionArea> Department of Ophthalmology and Visual Sciences, Washington University, St Louis, MO 63130</wicri:regionArea>
<wicri:noRegion>MO 63130</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Liu, Ying" sort="Liu, Ying" uniqKey="Liu Y" first="Ying" last="Liu">Ying Liu</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"> Department of Ophthalmology and Visual Sciences, Washington University, St Louis, MO 63130, USA.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">États-Unis</country>
<wicri:regionArea> Department of Ophthalmology and Visual Sciences, Washington University, St Louis, MO 63130</wicri:regionArea>
<wicri:noRegion>MO 63130</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kaartinen, Vesa" sort="Kaartinen, Vesa" uniqKey="Kaartinen V" first="Vesa" last="Kaartinen">Vesa Kaartinen</name>
<affiliation wicri:level="4"><nlm:aff id="aff3"> Developmental Biology Program, Childrens Hospital Los Angeles, Departments of Pathology and Surgery, Keck School of Medicine, University of Southern California, Los Angeles, CA 90027, USA.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">États-Unis</country>
<wicri:regionArea> Developmental Biology Program, Childrens Hospital Los Angeles, Departments of Pathology and Surgery, Keck School of Medicine, University of Southern California, Los Angeles, CA 90027</wicri:regionArea>
<orgName type="university">Université de Californie du Sud</orgName>
<placeName><settlement type="city">Los Angeles</settlement>
<region type="state">Californie</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Mishina, Yuji" sort="Mishina, Yuji" uniqKey="Mishina Y" first="Yuji" last="Mishina">Yuji Mishina</name>
<affiliation wicri:level="1"><nlm:aff id="aff4"> Molecular Developmental Biology Group, Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">États-Unis</country>
<wicri:regionArea> Molecular Developmental Biology Group, Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709</wicri:regionArea>
<wicri:noRegion>NC 27709</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Deng, Chu Xia" sort="Deng, Chu Xia" uniqKey="Deng C" first="Chu-Xia" last="Deng">Chu-Xia Deng</name>
<affiliation wicri:level="1"><nlm:aff id="aff5"> Genetics of Development and Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">États-Unis</country>
<wicri:regionArea> Genetics of Development and Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892</wicri:regionArea>
<wicri:noRegion>MD 20892</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Umans, Lieve" sort="Umans, Lieve" uniqKey="Umans L" first="Lieve" last="Umans">Lieve Umans</name>
<affiliation wicri:level="1"><nlm:aff id="aff6"> Laboratory Molecular Biology (Celgen), Department for Molecular and Developmental Genetics, VIB, B-3000 Leuven, Belgium.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">Belgique</country>
<wicri:regionArea> Laboratory Molecular Biology (Celgen), Department for Molecular and Developmental Genetics, VIB, B-3000 Leuven</wicri:regionArea>
<wicri:noRegion>B-3000 Leuven</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff7"> Laboratory Molecular Biology (Celgen), Center for Human Genetics, KU Leuven, B-3000 Leuven, Belgium.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">Belgique</country>
<wicri:regionArea> Laboratory Molecular Biology (Celgen), Center for Human Genetics, KU Leuven, B-3000 Leuven</wicri:regionArea>
<wicri:noRegion>B-3000 Leuven</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zwijsen, An" sort="Zwijsen, An" uniqKey="Zwijsen A" first="An" last="Zwijsen">An Zwijsen</name>
<affiliation wicri:level="1"><nlm:aff id="aff6"> Laboratory Molecular Biology (Celgen), Department for Molecular and Developmental Genetics, VIB, B-3000 Leuven, Belgium.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">Belgique</country>
<wicri:regionArea> Laboratory Molecular Biology (Celgen), Department for Molecular and Developmental Genetics, VIB, B-3000 Leuven</wicri:regionArea>
<wicri:noRegion>B-3000 Leuven</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff7"> Laboratory Molecular Biology (Celgen), Center for Human Genetics, KU Leuven, B-3000 Leuven, Belgium.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">Belgique</country>
<wicri:regionArea> Laboratory Molecular Biology (Celgen), Center for Human Genetics, KU Leuven, B-3000 Leuven</wicri:regionArea>
<wicri:noRegion>B-3000 Leuven</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Roberts, Anita B" sort="Roberts, Anita B" uniqKey="Roberts A" first="Anita B." last="Roberts">Anita B. Roberts</name>
<affiliation wicri:level="1"><nlm:aff id="aff8"> Laboratory of Cell Regulation and Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">États-Unis</country>
<wicri:regionArea> Laboratory of Cell Regulation and Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892</wicri:regionArea>
<wicri:noRegion>MD 20892</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Beebe, David C" sort="Beebe, David C" uniqKey="Beebe D" first="David C." last="Beebe">David C. Beebe</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"> Department of Ophthalmology and Visual Sciences, Washington University, St Louis, MO 63130, USA.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">États-Unis</country>
<wicri:regionArea> Department of Ophthalmology and Visual Sciences, Washington University, St Louis, MO 63130</wicri:regionArea>
<wicri:noRegion>MO 63130</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff2"> Department of Cell Biology and Physiology, Washington University, St Louis, MO 63130, USA.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">États-Unis</country>
<wicri:regionArea> Department of Cell Biology and Physiology, Washington University, St Louis, MO 63130</wicri:regionArea>
<wicri:noRegion>MO 63130</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">19369394</idno>
<idno type="pmc">2673764</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2673764</idno>
<idno type="RBID">PMC:2673764</idno>
<idno type="doi">10.1242/dev.034082</idno>
<date when="2009">2009</date>
<idno type="wicri:Area/Pmc/Corpus">001750</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001750</idno>
<idno type="wicri:Area/Pmc/Curation">001749</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001749</idno>
<idno type="wicri:Area/Pmc/Checkpoint">003739</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">003739</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="wicri:Area/PubMed/Corpus">002F04</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002F04</idno>
<idno type="wicri:Area/PubMed/Curation">002F04</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002F04</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002F04</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002F04</idno>
<idno type="wicri:Area/Ncbi/Merge">003352</idno>
<idno type="wicri:Area/Ncbi/Curation">003352</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">003352</idno>
<idno type="wicri:doubleKey">0950-1991:2009:Huang J:fgf:regulated:bmp</idno>
<idno type="wicri:Area/Main/Merge">006364</idno>
<idno type="wicri:Area/Main/Curation">006288</idno>
<idno type="wicri:Area/Main/Exploration">006288</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">FGF-regulated BMP signaling is required for eyelid closure and to specify
 conjunctival epithelial cell fate</title>
<author><name sortKey="Huang, Jie" sort="Huang, Jie" uniqKey="Huang J" first="Jie" last="Huang">Jie Huang</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"> Department of Ophthalmology and Visual Sciences, Washington University, St Louis, MO 63130, USA.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">États-Unis</country>
<wicri:regionArea> Department of Ophthalmology and Visual Sciences, Washington University, St Louis, MO 63130</wicri:regionArea>
<wicri:noRegion>MO 63130</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Dattilo, Lisa K" sort="Dattilo, Lisa K" uniqKey="Dattilo L" first="Lisa K." last="Dattilo">Lisa K. Dattilo</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"> Department of Ophthalmology and Visual Sciences, Washington University, St Louis, MO 63130, USA.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">États-Unis</country>
<wicri:regionArea> Department of Ophthalmology and Visual Sciences, Washington University, St Louis, MO 63130</wicri:regionArea>
<wicri:noRegion>MO 63130</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Rajagopal, Ramya" sort="Rajagopal, Ramya" uniqKey="Rajagopal R" first="Ramya" last="Rajagopal">Ramya Rajagopal</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"> Department of Ophthalmology and Visual Sciences, Washington University, St Louis, MO 63130, USA.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">États-Unis</country>
<wicri:regionArea> Department of Ophthalmology and Visual Sciences, Washington University, St Louis, MO 63130</wicri:regionArea>
<wicri:noRegion>MO 63130</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Liu, Ying" sort="Liu, Ying" uniqKey="Liu Y" first="Ying" last="Liu">Ying Liu</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"> Department of Ophthalmology and Visual Sciences, Washington University, St Louis, MO 63130, USA.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">États-Unis</country>
<wicri:regionArea> Department of Ophthalmology and Visual Sciences, Washington University, St Louis, MO 63130</wicri:regionArea>
<wicri:noRegion>MO 63130</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kaartinen, Vesa" sort="Kaartinen, Vesa" uniqKey="Kaartinen V" first="Vesa" last="Kaartinen">Vesa Kaartinen</name>
<affiliation wicri:level="4"><nlm:aff id="aff3"> Developmental Biology Program, Childrens Hospital Los Angeles, Departments of Pathology and Surgery, Keck School of Medicine, University of Southern California, Los Angeles, CA 90027, USA.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">États-Unis</country>
<wicri:regionArea> Developmental Biology Program, Childrens Hospital Los Angeles, Departments of Pathology and Surgery, Keck School of Medicine, University of Southern California, Los Angeles, CA 90027</wicri:regionArea>
<orgName type="university">Université de Californie du Sud</orgName>
<placeName><settlement type="city">Los Angeles</settlement>
<region type="state">Californie</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Mishina, Yuji" sort="Mishina, Yuji" uniqKey="Mishina Y" first="Yuji" last="Mishina">Yuji Mishina</name>
<affiliation wicri:level="1"><nlm:aff id="aff4"> Molecular Developmental Biology Group, Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">États-Unis</country>
<wicri:regionArea> Molecular Developmental Biology Group, Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709</wicri:regionArea>
<wicri:noRegion>NC 27709</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Deng, Chu Xia" sort="Deng, Chu Xia" uniqKey="Deng C" first="Chu-Xia" last="Deng">Chu-Xia Deng</name>
<affiliation wicri:level="1"><nlm:aff id="aff5"> Genetics of Development and Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">États-Unis</country>
<wicri:regionArea> Genetics of Development and Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892</wicri:regionArea>
<wicri:noRegion>MD 20892</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Umans, Lieve" sort="Umans, Lieve" uniqKey="Umans L" first="Lieve" last="Umans">Lieve Umans</name>
<affiliation wicri:level="1"><nlm:aff id="aff6"> Laboratory Molecular Biology (Celgen), Department for Molecular and Developmental Genetics, VIB, B-3000 Leuven, Belgium.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">Belgique</country>
<wicri:regionArea> Laboratory Molecular Biology (Celgen), Department for Molecular and Developmental Genetics, VIB, B-3000 Leuven</wicri:regionArea>
<wicri:noRegion>B-3000 Leuven</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff7"> Laboratory Molecular Biology (Celgen), Center for Human Genetics, KU Leuven, B-3000 Leuven, Belgium.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">Belgique</country>
<wicri:regionArea> Laboratory Molecular Biology (Celgen), Center for Human Genetics, KU Leuven, B-3000 Leuven</wicri:regionArea>
<wicri:noRegion>B-3000 Leuven</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zwijsen, An" sort="Zwijsen, An" uniqKey="Zwijsen A" first="An" last="Zwijsen">An Zwijsen</name>
<affiliation wicri:level="1"><nlm:aff id="aff6"> Laboratory Molecular Biology (Celgen), Department for Molecular and Developmental Genetics, VIB, B-3000 Leuven, Belgium.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">Belgique</country>
<wicri:regionArea> Laboratory Molecular Biology (Celgen), Department for Molecular and Developmental Genetics, VIB, B-3000 Leuven</wicri:regionArea>
<wicri:noRegion>B-3000 Leuven</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff7"> Laboratory Molecular Biology (Celgen), Center for Human Genetics, KU Leuven, B-3000 Leuven, Belgium.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">Belgique</country>
<wicri:regionArea> Laboratory Molecular Biology (Celgen), Center for Human Genetics, KU Leuven, B-3000 Leuven</wicri:regionArea>
<wicri:noRegion>B-3000 Leuven</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Roberts, Anita B" sort="Roberts, Anita B" uniqKey="Roberts A" first="Anita B." last="Roberts">Anita B. Roberts</name>
<affiliation wicri:level="1"><nlm:aff id="aff8"> Laboratory of Cell Regulation and Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">États-Unis</country>
<wicri:regionArea> Laboratory of Cell Regulation and Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892</wicri:regionArea>
<wicri:noRegion>MD 20892</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Beebe, David C" sort="Beebe, David C" uniqKey="Beebe D" first="David C." last="Beebe">David C. Beebe</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"> Department of Ophthalmology and Visual Sciences, Washington University, St Louis, MO 63130, USA.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">États-Unis</country>
<wicri:regionArea> Department of Ophthalmology and Visual Sciences, Washington University, St Louis, MO 63130</wicri:regionArea>
<wicri:noRegion>MO 63130</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff2"> Department of Cell Biology and Physiology, Washington University, St Louis, MO 63130, USA.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">États-Unis</country>
<wicri:regionArea> Department of Cell Biology and Physiology, Washington University, St Louis, MO 63130</wicri:regionArea>
<wicri:noRegion>MO 63130</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Development (Cambridge, England)</title>
<idno type="ISSN">0950-1991</idno>
<idno type="eISSN">1477-9129</idno>
<imprint><date when="2009">2009</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Animals, Newborn</term>
<term>Bone Morphogenetic Protein 4 (biosynthesis)</term>
<term>Bone Morphogenetic Protein Receptors, Type I (genetics)</term>
<term>Bone Morphogenetic Proteins (physiology)</term>
<term>Cell Differentiation (physiology)</term>
<term>Conjunctiva (abnormalities)</term>
<term>Conjunctiva (cytology)</term>
<term>Conjunctiva (embryology)</term>
<term>Conjunctiva (growth & development)</term>
<term>Epithelial Cells (cytology)</term>
<term>Epithelial Cells (metabolism)</term>
<term>Eyelids (abnormalities)</term>
<term>Eyelids (embryology)</term>
<term>Eyelids (growth & development)</term>
<term>Fibroblast Growth Factors (physiology)</term>
<term>Forkhead Transcription Factors (metabolism)</term>
<term>Hair Follicle (embryology)</term>
<term>Hair Follicle (growth & development)</term>
<term>Hedgehog Proteins (metabolism)</term>
<term>JNK Mitogen-Activated Protein Kinases (metabolism)</term>
<term>Mice</term>
<term>Mice, Knockout</term>
<term>Receptor, Fibroblast Growth Factor, Type 2 (genetics)</term>
<term>Receptor, Fibroblast Growth Factor, Type 2 (metabolism)</term>
<term>Signal Transduction</term>
<term>Smad Proteins, Receptor-Regulated (genetics)</term>
<term>Smad4 Protein (genetics)</term>
<term>Smad4 Protein (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Animaux nouveau-nés</term>
<term>Cellules épithéliales (cytologie)</term>
<term>Cellules épithéliales (métabolisme)</term>
<term>Conjonctive (croissance et développement)</term>
<term>Conjonctive (cytologie)</term>
<term>Conjonctive (embryologie)</term>
<term>Conjonctive (malformations)</term>
<term>Différenciation cellulaire (physiologie)</term>
<term>Facteurs de croissance fibroblastique (physiologie)</term>
<term>Facteurs de transcription Forkhead (métabolisme)</term>
<term>Follicule pileux (croissance et développement)</term>
<term>Follicule pileux (embryologie)</term>
<term>JNK Mitogen-Activated Protein Kinases (métabolisme)</term>
<term>Paupières (croissance et développement)</term>
<term>Paupières (embryologie)</term>
<term>Paupières (malformations)</term>
<term>Protéine Smad-4 (génétique)</term>
<term>Protéine Smad-4 (métabolisme)</term>
<term>Protéine morphogénétique osseuse de type 4 (biosynthèse)</term>
<term>Protéines Hedgehog (métabolisme)</term>
<term>Protéines Smad régulées par les récepteurs (génétique)</term>
<term>Protéines morphogénétiques osseuses (physiologie)</term>
<term>Récepteur FGFR2 (génétique)</term>
<term>Récepteur FGFR2 (métabolisme)</term>
<term>Récepteurs de la protéine morphogénique osseuse de type I (génétique)</term>
<term>Souris</term>
<term>Souris knockout</term>
<term>Transduction du signal</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Bone Morphogenetic Protein 4</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Bone Morphogenetic Protein Receptors, Type I</term>
<term>Receptor, Fibroblast Growth Factor, Type 2</term>
<term>Smad Proteins, Receptor-Regulated</term>
<term>Smad4 Protein</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Forkhead Transcription Factors</term>
<term>Hedgehog Proteins</term>
<term>JNK Mitogen-Activated Protein Kinases</term>
<term>Receptor, Fibroblast Growth Factor, Type 2</term>
<term>Smad4 Protein</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Bone Morphogenetic Proteins</term>
<term>Fibroblast Growth Factors</term>
</keywords>
<keywords scheme="MESH" qualifier="abnormalities" xml:lang="en"><term>Conjunctiva</term>
<term>Eyelids</term>
</keywords>
<keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Protéine morphogénétique osseuse de type 4</term>
</keywords>
<keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Conjonctive</term>
<term>Follicule pileux</term>
<term>Paupières</term>
</keywords>
<keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Cellules épithéliales</term>
<term>Conjonctive</term>
</keywords>
<keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Conjunctiva</term>
<term>Epithelial Cells</term>
</keywords>
<keywords scheme="MESH" qualifier="embryologie" xml:lang="fr"><term>Conjonctive</term>
<term>Follicule pileux</term>
<term>Paupières</term>
</keywords>
<keywords scheme="MESH" qualifier="embryology" xml:lang="en"><term>Conjunctiva</term>
<term>Eyelids</term>
<term>Hair Follicle</term>
</keywords>
<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Conjunctiva</term>
<term>Eyelids</term>
<term>Hair Follicle</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéine Smad-4</term>
<term>Protéines Smad régulées par les récepteurs</term>
<term>Récepteur FGFR2</term>
<term>Récepteurs de la protéine morphogénique osseuse de type I</term>
</keywords>
<keywords scheme="MESH" qualifier="malformations" xml:lang="fr"><term>Conjonctive</term>
<term>Paupières</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Epithelial Cells</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cellules épithéliales</term>
<term>Facteurs de transcription Forkhead</term>
<term>JNK Mitogen-Activated Protein Kinases</term>
<term>Protéine Smad-4</term>
<term>Protéines Hedgehog</term>
<term>Récepteur FGFR2</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Différenciation cellulaire</term>
<term>Facteurs de croissance fibroblastique</term>
<term>Protéines morphogénétiques osseuses</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Cell Differentiation</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Animals, Newborn</term>
<term>Mice</term>
<term>Mice, Knockout</term>
<term>Signal Transduction</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Animaux nouveau-nés</term>
<term>Souris</term>
<term>Souris knockout</term>
<term>Transduction du signal</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><title>Summary</title>
<p>There are conflicting reports about whether BMP signaling is required for
 eyelid closure during fetal development. This question was addressed using
 mice deficient in BMP or TGFβ signaling in prospective eyelid and
 conjunctival epithelial cells. Genes encoding two type I BMP receptors, the
 type II TGFβ receptor, two BMP- or two TGFβ-activated R-Smads or the
 co-Smad Smad4 were deleted from the ocular surface ectoderm using Cre
 recombinase. Only mice with deletion of components of the BMP pathway had an
 `eyelid open at birth' phenotype. Mice lacking <italic>Fgf10</italic> or
 <italic>Fgfr2</italic> also have open eyelids at birth. To better understand the
 pathways that regulate BMP expression and function during eyelid development,
 we localized BMPs and BMP signaling intermediates in <italic>Fgfr2</italic> and
 <italic>Smad4</italic> conditional knockout (CKO) mice. We found that Fgfr2 was
 required for the expression of <italic>Bmp4</italic>, the normal distribution of Shh
 signaling and for preserving the differentiation of the conjunctival
 epithelium. FGF signaling also promoted the expression of the Wnt antagonist
 Sfrp1 and suppressed Wnt signaling in the prospective eyelid epithelial cells,
 independently of BMP function. Transcripts encoding Foxc1 and Foxc2, which
 were previously shown to be necessary for eyelid closure, were not detectable
 in <italic>Smad4<sup>CKO</sup>
</italic> animals. c-Jun, another key regulator of
 eyelid closure, was present and phosphorylated in eyelid periderm cells at the
 time of fusion, but failed to translocate to the nucleus in the absence of BMP
 function. <italic>Smad4<sup>CKO</sup>
</italic> mice also showed premature
 differentiation of the conjunctival epithelium, conjunctival hyperplasia and
 the acquisition of epidermal characteristics, including formation of an
 ectopic row of hair follicles in place of the Meibomian glands. A second row
 of eyelashes is a feature of human lymphedema-distichiasis syndrome, which is
 associated with mutations in FOXC2.</p>
</div>
</front>
</TEI>
<affiliations><list><country><li>Belgique</li>
<li>États-Unis</li>
</country>
<region><li>Californie</li>
</region>
<settlement><li>Los Angeles</li>
</settlement>
<orgName><li>Université de Californie du Sud</li>
</orgName>
</list>
<tree><country name="États-Unis"><noRegion><name sortKey="Huang, Jie" sort="Huang, Jie" uniqKey="Huang J" first="Jie" last="Huang">Jie Huang</name>
</noRegion>
<name sortKey="Beebe, David C" sort="Beebe, David C" uniqKey="Beebe D" first="David C." last="Beebe">David C. Beebe</name>
<name sortKey="Beebe, David C" sort="Beebe, David C" uniqKey="Beebe D" first="David C." last="Beebe">David C. Beebe</name>
<name sortKey="Dattilo, Lisa K" sort="Dattilo, Lisa K" uniqKey="Dattilo L" first="Lisa K." last="Dattilo">Lisa K. Dattilo</name>
<name sortKey="Deng, Chu Xia" sort="Deng, Chu Xia" uniqKey="Deng C" first="Chu-Xia" last="Deng">Chu-Xia Deng</name>
<name sortKey="Kaartinen, Vesa" sort="Kaartinen, Vesa" uniqKey="Kaartinen V" first="Vesa" last="Kaartinen">Vesa Kaartinen</name>
<name sortKey="Liu, Ying" sort="Liu, Ying" uniqKey="Liu Y" first="Ying" last="Liu">Ying Liu</name>
<name sortKey="Mishina, Yuji" sort="Mishina, Yuji" uniqKey="Mishina Y" first="Yuji" last="Mishina">Yuji Mishina</name>
<name sortKey="Rajagopal, Ramya" sort="Rajagopal, Ramya" uniqKey="Rajagopal R" first="Ramya" last="Rajagopal">Ramya Rajagopal</name>
<name sortKey="Roberts, Anita B" sort="Roberts, Anita B" uniqKey="Roberts A" first="Anita B." last="Roberts">Anita B. Roberts</name>
</country>
<country name="Belgique"><noRegion><name sortKey="Umans, Lieve" sort="Umans, Lieve" uniqKey="Umans L" first="Lieve" last="Umans">Lieve Umans</name>
</noRegion>
<name sortKey="Umans, Lieve" sort="Umans, Lieve" uniqKey="Umans L" first="Lieve" last="Umans">Lieve Umans</name>
<name sortKey="Zwijsen, An" sort="Zwijsen, An" uniqKey="Zwijsen A" first="An" last="Zwijsen">An Zwijsen</name>
<name sortKey="Zwijsen, An" sort="Zwijsen, An" uniqKey="Zwijsen A" first="An" last="Zwijsen">An Zwijsen</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 006288 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 006288 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Sante
   |area=    LymphedemaV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     PMC:2673764
   |texte=   FGF-regulated BMP signaling is required for eyelid closure and to specify
 conjunctival epithelial cell fate
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:19369394" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a LymphedemaV1

This area was generated with Dilib version V0.6.31.
Data generation: Sat Nov 4 17:40:35 2017. Site generation: Tue Feb 13 16:42:16 2024

Serveur d'exploration sur le lymphœdème

FGF-regulated BMP signaling is required for eyelid closure and to specify conjunctival epithelial cell fate

FGF-regulated BMP signaling is required for eyelid closure and to specify conjunctival epithelial cell fate

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration sur le lymphœdème
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.